Lipolysis During Hypoglycemia in Patients with Type 1 Diabetes Mellitus

The broad, long-term objective of this proposal is the application of minimally invasive and noninvasive state-of-the-art techniques for assessment of whole-body and regional metabolism to investigate the physiological and pathophysiological mechanisms that regulate hypoglycemic counterregulation. It is our aim to apply stable isotope dilution techniques (to assess systemic and local lipolysis) with the unique combination of open-flow microperfusion (to characterize the microenvironment in adipose and muscle interstitial fluid), indirect calorimetry (for the measurement of fuel oxidation), and 1H-NMR spectroscopy (to measure intramuscular lipid content) to examine lipolysis during prolonged moderate hypoglycemia in well-controlled patients with type 1 diabetes mellitus and nondiabetic subjects.