Abstract
Photo- and biocatalysis show many advantages as more sustainable solutions for the production of fine chemicals. In an effort to combine the benefits and the knowledge of both these areas, a continuous photobiocatalytic setup was designed and optimized to carry out whole-cell biotransformations within cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM from B. subtilis. The effect of the light intensity and flow rate on the specific activity in the stereoselective reduction of 2-methyl maleimide was investigated via a design-of-experiments approach. The cell density in the setup was further increased at the optimal operating conditions without loss in specific activity, demonstrating that the higher surface area/volume ratio in the coil reactor improved the illumination efficiency of the process. Furthermore, different reactor designs were compared, proving that the presented approach was the most cost- and time-effective solution for intensifying photobiotransformations within cyanobacterial cells.
Originalsprache | englisch |
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Aufsatznummer | e202201468 |
Fachzeitschrift | ChemSusChem |
Jahrgang | 15 |
Ausgabenummer | 22 |
Frühes Online-Datum | 7 Sept. 2022 |
DOIs | |
Publikationsstatus | Veröffentlicht - 22 Nov. 2022 |
ASJC Scopus subject areas
- Allgemeine Energie
- Allgemeine chemische Verfahrenstechnik
- Allgemeine Materialwissenschaften
- Umweltchemie