TY - JOUR
T1 - Development of a generic process model for membrane adsorption
AU - van Beijeren, Peter
AU - Kreis, Peter
AU - Zeiner, Tim
PY - 2013/6/1
Y1 - 2013/6/1
N2 - In this work, a generic model describing the dynamic adsorption behaviour of proteins on membrane adsorbers over complete purification cycles under consideration of module geometry and of the interaction between multiple transport mechanisms is developed.A general rate model for membrane adsorption, in which the interaction between multiple phenomena, like mass transfer and adsorption kinetics are considered, is formulated. Hereby, the implemented isotherms describe the influence of eluting agents on the adsorption behaviour, so that complete purification cycle (loading, washing and elution operation) can be simulated.Using the developed model the theoretical influence of relevant transport phenomena, operating conditions and process scale on affinity and ion exchange membrane adsorption of proteins are investigated. An example on ion exchange membrane adsorption illustrates the possibility to predict scale up effects occurring in configurations of multiple membrane adsorber modules. The obtained simulation results are in accordance with experimental observations reported in literature.
AB - In this work, a generic model describing the dynamic adsorption behaviour of proteins on membrane adsorbers over complete purification cycles under consideration of module geometry and of the interaction between multiple transport mechanisms is developed.A general rate model for membrane adsorption, in which the interaction between multiple phenomena, like mass transfer and adsorption kinetics are considered, is formulated. Hereby, the implemented isotherms describe the influence of eluting agents on the adsorption behaviour, so that complete purification cycle (loading, washing and elution operation) can be simulated.Using the developed model the theoretical influence of relevant transport phenomena, operating conditions and process scale on affinity and ion exchange membrane adsorption of proteins are investigated. An example on ion exchange membrane adsorption illustrates the possibility to predict scale up effects occurring in configurations of multiple membrane adsorber modules. The obtained simulation results are in accordance with experimental observations reported in literature.
KW - Affinity membrane adsorption
KW - Ion exchange membrane adsorption
KW - Membrane chromatography
KW - Model based scale up
KW - Process simulation
UR - http://www.scopus.com/inward/record.url?scp=84875762627&partnerID=8YFLogxK
U2 - 10.1016/j.compchemeng.2013.03.005
DO - 10.1016/j.compchemeng.2013.03.005
M3 - Article
AN - SCOPUS:84875762627
SN - 0098-1354
VL - 53
SP - 86
EP - 101
JO - Computers & Chemical Engineering
JF - Computers & Chemical Engineering
ER -