Abstract
We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87% inside of the same patient cohort for which the markers were developed and up to 81% in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.
Originalsprache | englisch |
---|---|
Seiten (von - bis) | 80-88 |
Seitenumfang | 9 |
Fachzeitschrift | Journal of Biotechnology |
Jahrgang | 310 |
DOIs | |
Publikationsstatus | Veröffentlicht - 20 Feb. 2020 |
ASJC Scopus subject areas
- Biochemie, Genetik und Molekularbiologie (sonstige)
- Biotechnology
- Angewandte Mikrobiologie und Biotechnologie
- Bioengineering
Fields of Expertise
- Human- & Biotechnology
- Information, Communication & Computing
Treatment code (Nähere Zuordnung)
- Application