Abstract
In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.
Originalsprache | englisch |
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Seiten (von - bis) | 349-354 |
Seitenumfang | 6 |
Fachzeitschrift | Chemical Engineering Transactions |
Jahrgang | 27 |
DOIs | |
Publikationsstatus | Veröffentlicht - 2012 |
ASJC Scopus subject areas
- Allgemeine chemische Verfahrenstechnik