Abstract
Functional coatings with amino groups are used in a wide range of biochemistry-related applications. A technological platform that takes advantage of the affinity between amino-functionalized coatings and biomolecules is the DNA microarray methodology. Reliability and reproducibility of the microarray data strongly depend on the quality of the substrate; therefore, a proper awareness of how the storage conditions affect the amino-functionalized coatings is necessary. In this work we have studied the influence of different relative humidity levels on aminomethyl- silane coatings prepared via sol-gel methodology. Drops of a buffer solution containing a luminescent dye have been deposited (or spotted) on the coating; the dye molecules react with the amino-groups and leave circular luminescent marks (spots) on the substrate surface. Shape and luminescence of the spots, as well as background signals, have been monitored using a microarray laser scanner. With the proposed protocol we have measured the changes of these variables due to different storage conditions. FT-IR measurements have been performed to investigate the related chemistry changes.
Originalsprache | englisch |
---|---|
Seiten (von - bis) | 246-253 |
Seitenumfang | 8 |
Fachzeitschrift | Journal of Sol-Gel Science and Technology |
Jahrgang | 60 |
Ausgabenummer | 3 |
DOIs | |
Publikationsstatus | Veröffentlicht - Dez. 2011 |
Extern publiziert | Ja |
ASJC Scopus subject areas
- Chemie (insg.)
- Physik der kondensierten Materie
- Biomaterialien
- Keramische und Verbundwerkstoffe
- Elektronische, optische und magnetische Materialien
- Werkstoffchemie