TY - JOUR
T1 - NR4A3 Suppresses Lymphomagenesis through Induction of Proapoptotic Genes
AU - Deutsch, Alexander J A
AU - Rinner, Beate
AU - Pichler, Martin
AU - Prochazka, Katharina
AU - Pansy, Katrin
AU - Bischof, Marco
AU - Fechter, Karoline
AU - Hatzl, Stefan
AU - Feichtinger, Julia
AU - Wenzl, Kerstin
AU - Frisch, Marie-Therese
AU - Stiegelbauer, Verena
AU - Prokesch, Andreas
AU - Krogsdam, Anne
AU - Sill, Heinz
AU - Thallinger, Gerhard G
AU - Greinix, Hildegard T
AU - Wang, Chenguang
AU - Beham-Schmid, Christine
AU - Neumeister, Peter
N1 - ©2017 American Association for Cancer Research.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Nuclear orphan receptor NR4A1 exerts an essential tumor suppressor function in aggressive lymphomas. In this study, we investigated the hypothesized contribution of the related NR4A family member NR4A3 to lymphomagenesis. In aggressive lymphoma patients, low expression of NR4A3 was associated with poor survival. Ectopic expression or pharmacological activation of NR4A3 in lymphoma cell lines led to a significantly higher proportion of apoptotic cells. In a mouse NSG xenograft model of lymphoma (stably transduced SuDHL4 cells), NR4A3 expression abrogated tumor growth, compared with vector control and uninduced cells that formed massive tumors. Transcript analysis of four different aggressive lymphoma cell lines overexpressing either NR4A3 or NR4A1 revealed that apoptosis was driven similarly by induction of BAK, Puma, BIK, BIM, BID, and Trail. Overall, our results showed that NR4A3 possesses robust tumor suppressor functions of similar impact to NR4A1 in aggressive lymphomas. Cancer Res; 77(9); 2375-86. ©2017 AACR.
AB - Nuclear orphan receptor NR4A1 exerts an essential tumor suppressor function in aggressive lymphomas. In this study, we investigated the hypothesized contribution of the related NR4A family member NR4A3 to lymphomagenesis. In aggressive lymphoma patients, low expression of NR4A3 was associated with poor survival. Ectopic expression or pharmacological activation of NR4A3 in lymphoma cell lines led to a significantly higher proportion of apoptotic cells. In a mouse NSG xenograft model of lymphoma (stably transduced SuDHL4 cells), NR4A3 expression abrogated tumor growth, compared with vector control and uninduced cells that formed massive tumors. Transcript analysis of four different aggressive lymphoma cell lines overexpressing either NR4A3 or NR4A1 revealed that apoptosis was driven similarly by induction of BAK, Puma, BIK, BIM, BID, and Trail. Overall, our results showed that NR4A3 possesses robust tumor suppressor functions of similar impact to NR4A1 in aggressive lymphomas. Cancer Res; 77(9); 2375-86. ©2017 AACR.
KW - Animals
KW - Apoptosis
KW - Carcinogenesis
KW - Cell Line, Tumor
KW - Cell Proliferation
KW - DNA-Binding Proteins
KW - Disease-Free Survival
KW - Female
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Kaplan-Meier Estimate
KW - Lymphoma
KW - Male
KW - Mice
KW - Receptors, Steroid
KW - Receptors, Thyroid Hormone
KW - Xenograft Model Antitumor Assays
KW - Journal Article
U2 - 10.1158/0008-5472.CAN-16-2320
DO - 10.1158/0008-5472.CAN-16-2320
M3 - Article
C2 - 28249906
SN - 1538-7445
VL - 77
SP - 2375
EP - 2386
JO - Cancer Research
JF - Cancer Research
IS - 9
ER -