Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris

Claudia Rinnofner*, Michael Felber, Harald Pichler

*Korrespondierende/r Autor/-in für diese Arbeit

Publikation: Beitrag in Buch/Bericht/KonferenzbandBeitrag in Buch/BerichtBegutachtung

Abstract

Within the last two decades, the methylotrophic yeast Pichia pastoris (Komagataella phaffii) has become an important alternative to E. coli or mammalian cell lines for the production of recombinant proteins. Easy handling, strong promoters, and high cell density cultivations as well as the capability of posttranslational modifications are some of the major benefits of this yeast. The high secretion capacity and low level of endogenously secreted proteins further promoted the rapid development of a versatile Pichia pastoris toolbox. This chapter reviews common and new “Pichia tools” and their specific features. Special focus is given to expression strains, such as different methanol utilization, protease-deficient or glycoengineered strains, combined with application highlights. Different promoters and signal sequences are also discussed.
Originalspracheenglisch
TitelYeast Metabolic Engineering: Methods and Protocols
Redakteure/-innenValeria Mapelli, Maurizio Bettiga
ErscheinungsortNew York, U.S.A.
Herausgeber (Verlag)Springer
Kapitel6
Seiten79-112
Seitenumfang34
Auflage2nd
ISBN (elektronisch)978-1-0716-2399-2
ISBN (Print)978-1-0716-2398-5
DOIs
PublikationsstatusVeröffentlicht - 1 Juni 2022

Publikationsreihe

NameMethods in Molecular Biology
Herausgeber (Verlag)Humana Press
Band2513
ISSN (Print)1064-3745
ISSN (elektronisch)1940-6029

ASJC Scopus subject areas

  • Genetik
  • Molekularbiologie

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)
  • Popular Scientific
  • Review

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