Komagataella phaffii (widely known as P. pastoris) is a common protein expression host in academia and industry. However, most protein production is still performed with E. coli, filamentous fungi and tissue culture cell lines, such as CHO. P. pastoris in most cases is still the second choice for many labs. Exploring characteristic advantages of P. pastoris, this CD laboratory will lead to a better understanding of basic biology of this yeast and drive the transformation of P. pastoris to a first choice expression system. Research for innovative gene cloning/vector construction, efficient cell transformation and expression/secretion of recombinant proteins will enable new applications and make previous efforts economically successful.