Project Details
Description
The primary goal of this research is to investigate the relationship between the age of cells and the production of recombinant proteins in P. pastoris. A key approach to explore this relationship involves inducing cell cycle arrest through the synthesis of recombinant mating factors (MFa1 and MFa2). In parallel, we aim to develop an enhanced magnetic millifluidic fractionation method specifically for P. pastoris, to improve age selectivity and thus the efficiency of cultivations. We will also evaluate MOF-encapsulation for preserving rejuvenated and synchronized cells. By utilizing cell cycle-synchronized populations, we plan to identify the optimal stages for substrate uptake, such as methanol, glycerine, and sugar, leveraging insights from the parallel behavior between S. cerevisiae and P. pastoris in pheromone response and gene expression. This research is expected to significantly advance the productivity and efficiency of P. pastoris-based processes, deepening our understanding of how cell age affects recombinant protein synthesis, with far-reaching implications for various applications.
Status | Active |
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Effective start/end date | 1/09/24 → 31/08/27 |
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