A two years study reveals implications of feeding management and exposure to mycotoxins on udder health, performance, and fertility in dairy herds

We recently reported the ubiquitous occurrence of mycotoxins and their secondary metabolites in dairy rations and a substantial variation in the feeding management among Austrian dairy farms. The present study aimed to characterize to which extent these factors contribute to the fertility, udder health traits, and performance of dairy herds. During 2019 and 2020, we surveyed 100 dairy farms, visiting each farm 2 times and collecting data and feed samples. Data collection involved information on the main feed ingredients, nutrient composition, and the levels of mycotoxin and other metabolites in the diet. The annual fertility and milk data of the herds were obtained from the national reporting agency. Calving interval was the target criterion for fertility performance, whereas the percentage of primiparous and multiparous in the herd with somatic cell counts (SCC) above 200,000 cells/mL for impaired udder health. For each criterion, herds were classified into 3 groups: High/Long, Mid and Low/Short, with the cut-off corresponding to the < 25th and > 75th percentiles and the rest of the data, respectively. Accordingly, for the calving interval, the cut-offs for the Long and Short groups were ≥400 d and ≤380 d, for the udder health in primiparous cows were ≥20% and ≤8% of the herd, and for the udder health in multiparous cows were ≥35% and ≤20% % of the herd, respectively. Quantitative approaches were further performed to define potential risk factors in the herds. The High SCC group had higher dietary concentrations of enniatins (ENNs) (2.8 vs. 1.62 mg/cow/day), deoxynivalenol (DON) (4.91 vs. 2.3 mg/cow/day), culmorin (CUL) (9.48 vs. 5.72 mg/cow/day), beauvericin (BEA) (0.32 vs. 0.18 mg/cow/d) and siccanol (13.3 vs. 5.15 mg/cow/d), total Fusarium metabolites (42.8 vs. 23.2 mg/cow/d) and used more corn silage in the ration (26.9 vs. 17.3% diet DM) compared with the Low counterparts. BEA was the most substantial contributing variable among the Fusarium metabolites, as indicated by logistic regression and modeling analyses. Logistic analysis indicated that herds with high proportions of cows with milk fat-to-protein ratio >1.5 had an increased odds for a longer calving interval, which was found significant for primiparous cows (odds ratio = 5.5, 95% confidence interval (CI) = 1.65 - 21.7). As well, herds with high proportions of multiparous cows showing levels of milk urea nitrogen > 30 mg/dL had an increased odds for longer calving intervals (odds ratio = 2.96, CI = 1.22 - 7.87). In conclusion, the present findings suggest that dietary contamination of Fusarium mycotoxins (especially emerging ones), likely due to increased use of corn silage in the diet, seems to be a risk factor for impairing the udder health of primiparous cows. Mismatching dietary energy and protein supply of multiparous cows contributed to reduced herd fertility performance.