An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues

Anna Pukaluk; Heimo Wolinski; Christian Viertler; Peter Regitnig; Gerhard Holzapfel; Gerhard Sommer

doi:DOI 10.3217/978-3-85125-826-4

An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues

Anna Pukaluk^*, Heimo Wolinski, Christian Viertler, Peter Regitnig, Gerhard Holzapfel, Gerhard Sommer

^*Corresponding author for this work

Institute of Biomechanics (7190)

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › peer-review

Abstract

Knowledge of the interaction between the constituents of loaded aortic tissues is crucial to expand our understanding of load-bearing mechanisms in the aorta. We have therefore developed a procedure that enables simultaneous multi-photon microscopy imaging of collagen and elastin in human aortic tissue during the biaxial extension test. The microscopy images obtained were verified with the results of the histological staining. The mechanical response was also compared with findings from previously performed biaxial extension tests. The proposed pipeline has shown successful and has great potential for structural analysis of human aortic tissue.

Original language	English
Title of host publication	Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021
Editors	Gernot Müller-Putz, Christian Baumgartner
Place of Publication	Graz
Publisher	Verlag der Technischen Universität Graz
Pages	21-24
ISBN (Print)	978-3-85125-826-4
DOIs	https://doi.org/DOI 10.3217/978-3-85125-826-4
Publication status	Published - 2021
Event	Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021 - Graz, Austria Duration: 30 Sept 2021 → 1 Oct 2021

Conference

Conference	Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021
Country/Territory	Austria
City	Graz
Period	30/09/21 → 1/10/21

Keywords

human aorta
collagen
elastin
biaxial extension test
multi-photon microscopy

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DOI 10.3217/978-3-85125-826-4Licence: CC BY 4.0

Cite this

Pukaluk, A., Wolinski, H., Viertler, C., Regitnig, P., Holzapfel, G., & Sommer, G. (2021). An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues. In G. Müller-Putz, & C. Baumgartner (Eds.), Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021 (pp. 21-24). Verlag der Technischen Universität Graz. https://doi.org/DOI 10.3217/978-3-85125-826-4

An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues. / Pukaluk, Anna; Wolinski, Heimo; Viertler, Christian et al.
Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021. ed. / Gernot Müller-Putz; Christian Baumgartner. Graz: Verlag der Technischen Universität Graz, 2021. p. 21-24.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › peer-review

Pukaluk, A, Wolinski, H, Viertler, C, Regitnig, P, Holzapfel, G & Sommer, G 2021, An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues. in G Müller-Putz & C Baumgartner (eds), Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021. Verlag der Technischen Universität Graz, Graz, pp. 21-24, Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021, Graz, Austria, 30/09/21. https://doi.org/DOI 10.3217/978-3-85125-826-4

Pukaluk A, Wolinski H, Viertler C, Regitnig P, Holzapfel G , Sommer G. An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues. In Müller-Putz G, Baumgartner C, editors, Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021. Graz: Verlag der Technischen Universität Graz. 2021. p. 21-24 doi: DOI 10.3217/978-3-85125-826-4

Pukaluk, Anna ; Wolinski, Heimo ; Viertler, Christian et al. / An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues. Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021. editor / Gernot Müller-Putz ; Christian Baumgartner. Graz : Verlag der Technischen Universität Graz, 2021. pp. 21-24

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AU - Sommer, Gerhard

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AB - Knowledge of the interaction between the constituents of loaded aortic tissues is crucial to expand our understanding of load-bearing mechanisms in the aorta. We have therefore developed a procedure that enables simultaneous multi-photon microscopy imaging of collagen and elastin in human aortic tissue during the biaxial extension test. The microscopy images obtained were verified with the results of the histological staining. The mechanical response was also compared with findings from previously performed biaxial extension tests. The proposed pipeline has shown successful and has great potential for structural analysis of human aortic tissue.

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KW - collagen

KW - elastin

KW - biaxial extension test

KW - multi-photon microscopy

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DO - DOI 10.3217/978-3-85125-826-4

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SN - 978-3-85125-826-4

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BT - Proceedings of the Annual Meeting of the Austrian Society for Biomedical Engineering ÖGBMT2021

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Y2 - 30 September 2021 through 1 October 2021

ER -

An approach for visualization of the interaction between collagen and elastin in loaded human aortic tissues

Abstract

Conference

Keywords

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