@inbook{38f48f55fa854cfea6b7edf4c928a41e,
title = "Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli",
abstract = "In this chapter, we present a bidirectional promoter library toolbox to evaluate fast and efficiently the optimal conditions for the balanced co-expression of two target genes. As a proof-of-concept, we demonstrate the co-expression of CYP505x and the GroEL/ES complex, which resulted in noticeably elevated enzyme activity with one of the de-novo-designed promoters of the library. The new toolbox offers a straightforward one-pot cloning approach and is highly modular. As such, the method presented here should be of great interest to any gene co-expression study.",
keywords = "Bidirectional promoters, E. coli, GroEL/ES, One-pot library cloning, P450s",
author = "Julia Jodlbauer and Lukas Rieder and Anton Glieder and Birgit Wiltschi",
year = "2023",
doi = "10.1007/978-1-0716-2930-7_5",
language = "English",
volume = "2617",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "75--86",
booktitle = "Methods in molecular biology",
}