Bioinspired Antimicrobial Coatings from Peptide-Functionalized Liquid Crystalline Nanostructures

Mahsa Zabara, Qun Ren, Heinz Amenitsch, Stefan Salentinig*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Surface-associated microbial infections and contaminations are a major challenge in various fields including the food and health sectors. This study demonstrates the design of antimicrobial coatings based on the self-assembly of the food-grade amphiphilic lipid glycerol monooleate with the human cathelicidin-derived antimicrobial peptide LL-37. Structural properties of the coating and their alterations with composition were studied using advanced experimental methods including synchrotron grazing-incidence small-angle X-ray scattering and ellipsometry. The integration of the LL-37 and its potential release from the nanostructured films into the surrounding solution was characterized with confocal Raman microscopy. Additional biological evaluation studies with clinically relevant bacterial strains, namely,Pseudomonas aeruginosa(Gram-negative) andStaphylococcus aureus(Gram-positive), were performed to investigate the antimicrobial activity of the coatings. Significant killing activity of the coating was found against both bacterial strains. The presented findings contribute to the fundamental understanding of lipid-peptide self-assembly on the surface and may open up a promising strategy for designing simple, sustainable antimicrobial coatings for medical and food applications.

Original languageEnglish
Pages (from-to)5295-5303
Number of pages9
JournalACS Applied Bio Materials
Issue number6
Publication statusPublished - 21 Jun 2021


  • antimicrobial coatings
  • in situ characterization
  • nanostructured films
  • self-assembly
  • stimuli-responsive materials

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, medical

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)


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