Cationically rendered biopolymer surfaces for high protein affinity support matrices

Tamilselvan Mohan; Rupert Kargl; Ales Doliska; Stefan Köstler; Volker Ribitsch; Jure Marn; Stefan Spirk; Karin Stana-Kleinschek

doi:10.1039/C3CC46414H

Cationically rendered biopolymer surfaces for high protein affinity support matrices

Tamilselvan Mohan, Rupert Kargl, Ales Doliska, Stefan Köstler, Volker Ribitsch, Jure Marn, Stefan Spirk, Karin Stana-Kleinschek

Institute for Chemistry and Technology of Materials (6380)

Research output: Contribution to journal › Article › peer-review

Abstract

The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.

Original language	English
Pages (from-to)	11530-11532
Journal	Chemical Communications
Volume	49
DOIs	https://doi.org/10.1039/C3CC46414H
Publication status	Published - 2013

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Sustainable Systems

Treatment code (Nähere Zuordnung)

Basic - Fundamental (Grundlagenforschung)
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10.1039/C3CC46414H

Cite this

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title = "Cationically rendered biopolymer surfaces for high protein affinity support matrices",

abstract = "The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.",

author = "Tamilselvan Mohan and Rupert Kargl and Ales Doliska and Stefan K{\"o}stler and Volker Ribitsch and Jure Marn and Stefan Spirk and Karin Stana-Kleinschek",

year = "2013",

doi = "10.1039/C3CC46414H",

language = "English",

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pages = "11530--11532",

journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - Cationically rendered biopolymer surfaces for high protein affinity support matrices

AU - Mohan, Tamilselvan

AU - Kargl, Rupert

AU - Doliska, Ales

AU - Köstler, Stefan

AU - Ribitsch, Volker

AU - Marn, Jure

AU - Spirk, Stefan

AU - Stana-Kleinschek, Karin

PY - 2013

Y1 - 2013

N2 - The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.

AB - The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.

U2 - 10.1039/C3CC46414H

DO - 10.1039/C3CC46414H

M3 - Article

SN - 1364-548X

VL - 49

SP - 11530

EP - 11532

JO - Chemical Communications

JF - Chemical Communications

ER -

Cationically rendered biopolymer surfaces for high protein affinity support matrices

Abstract

Fields of Expertise

Treatment code (Nähere Zuordnung)

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