Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes

Daniel Schwendenwein, Anna K. Ressmann, Marcello Entner, Viktor Savic, Margit Winkler*, Florian Rudroff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and the development of a highly versatile high-throughput assay for the enzymatic reduction of carboxylic acids. We investigated a biocompatible metal-catalyzed synthesis for the preparation of α or β substituted cinnamic acid derivatives which were fed directly into the biocatalytic system. Subsequently, the target molecules were synthesized by an enzymatic cascade consisting of a carboxylate reduction, followed by the selective C-C double bond reduction catalyzed by appropriate enoate reductases. We investigated a biocompatible oxidative Heck protocol and combined it with cells expressing a carboxylic acid reductase from Neurospora crassa (NcCAR) and an ene reductase from Saccharomyces pastorianus for the production fragrance aldehydes
Original languageEnglish
Article number932
Issue number8
Publication statusPublished - Aug 2021


  • Carboxylic acid reductase
  • Chemo-enzymatic cascade
  • Ene reductase
  • Fragrance aldehydes
  • Heck-coupling

ASJC Scopus subject areas

  • Catalysis
  • Physical and Theoretical Chemistry

Fields of Expertise

  • Human- & Biotechnology

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