Abstract
In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and the development of a highly versatile high-throughput assay for the enzymatic reduction of carboxylic acids. We investigated a biocompatible metal-catalyzed synthesis for the preparation of α or β substituted cinnamic acid derivatives which were fed directly into the biocatalytic system. Subsequently, the target molecules were synthesized by an enzymatic cascade consisting of a carboxylate reduction, followed by the selective C-C double bond reduction catalyzed by appropriate enoate reductases. We investigated a biocompatible oxidative Heck protocol and combined it with cells expressing a carboxylic acid reductase from Neurospora crassa (NcCAR) and an ene reductase from Saccharomyces pastorianus for the production fragrance aldehydes
Original language | English |
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Article number | 932 |
Journal | Catalysts |
Volume | 11 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2021 |
Keywords
- Carboxylic acid reductase
- Chemo-enzymatic cascade
- Ene reductase
- Fragrance aldehydes
- Heck-coupling
ASJC Scopus subject areas
- Catalysis
- Physical and Theoretical Chemistry
Fields of Expertise
- Human- & Biotechnology