Abstract
Mineral oil aromatic hydrocarbons (MOAHs) include mutagenic and carcinogenic substances and are considered a potential health risk. Current methods address the total MOAH content but cannot address the actual toxicological hazard of individual components. This work presents a combined methodology closing those gaps: high-performance liquid chromatography (HPLC) coupled to gas chromatography with flame ionization detection was used to determine the MOAH content. To characterize present substance classes, comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry was applied. Preparative HPLC separated MOAHs into subgroups, which were tested with a miniaturized Ames test evaluating DNA reactivity of isolated fractions. Combining these methods allowed a correlation between present subgroups and DNA reactivity. The developed approach was applied to a mineral oil and distinguished between not DNA-reactive mono- and diaromatics and DNA-reactive tri- and polyaromatics, providing a proof of concept. Hereinafter, it will be applied to diverse sample matrices including mineral oils, food, and food contact materials.
Original language | English |
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Pages (from-to) | 16401–16409 |
Number of pages | 9 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 70 |
Issue number | 51 |
DOIs | |
Publication status | Published - 28 Dec 2022 |
Keywords
- MOAH, HPLC-GC-FID, GC × GC-ToF, Ames assay, miniaturized Ames assay
- GC × GC-ToF
- HPLC-GC-FID
- Ames assay
- miniaturized Ames assay
- MOAH
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)