Combining a Genetically Engineered Oxidase with Hydrogen-Bonded Organic Frameworks (HOFs) for Highly Efficient Biocomposites

Peter Wied, Francesco Carraro, Juan M Bolivar, Christian J Doonan*, Paolo Falcaro*, Bernd Nidetzky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Enzymes incorporated into hydrogen bonded organic frameworks (HOFs) via bottom-up synthesis are promising biocomposites for applications in catalysis and sensing. Here, we explored synthetic incorporation of d-amino acid oxidase (DAAO) with the metal-free tetraamidine/tetracarboxylate-based BioHOF-1 in water. N-terminal enzyme fusion with the positively charged module Zbasic2 strongly boosted the loading (2.5-fold; ~500 mg enzyme gmaterial-1) and the specific activity (6.5-fold; 23 U mg-1). The DAAO@BioHOF-1 composites showed superior activity with respect to every reported carrier for the same enzyme and excellent stability during catalyst recycling. Further, extension to other enzymes, including cytochrome P450 BM3 (used in the production high-value oxyfunctionalized compounds), points to the versatility of genetic engineering as a strategy for the preparation of biohybrid systems with unprecedented properties.

Original languageEnglish
Article numbere202117345
JournalAngewandte Chemie - International Edition
Volume61
Issue number16
Early online date17 Jan 2022
DOIs
Publication statusPublished - 11 Apr 2022

Keywords

  • Biocatalysis
  • Hydrogen-Bonded Organic Frameworks
  • Immobilization
  • Metal–Organic Frameworks
  • Porous Carrier

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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