TY - JOUR
T1 - Comparison of a fungal and a bacterial laccase for lignosulfonate polymerization
AU - Braunschmid, Verena
AU - Binder, Karin
AU - Fuerst, Sarah
AU - Subagia, Raditya
AU - Danner, Caroline
AU - Weber, Hedda
AU - Schwaiger, Nikolaus
AU - Nyanhongo, Gibson S.
AU - Ribitsch, Doris
AU - Guebitz, Georg M.
PY - 2021/10
Y1 - 2021/10
N2 - Lignin is the second most abundant biopolymer on earth and accrues in large amounts in the pulp- and paper industry. While currently lignins are mainly used for low-value applications such as energy production, recently the potential of laccases for upgrading lignins has been demonstrated. In this study, two laccases of fungal and bacterial origin were characterized regarding their potential to polymerize lignosulfonate. The laccases MaL1 from Melanocarpus albomyces, and SrLA from Streptomyces rochei were heterologously expressed and showed typical characteristics of laccases, like acidic pH optima for ABTS at pH 4 and 5, respectively, and temperature optima at 50 °C and 80 °C. Polymerization of lignosulfonate with MaL1 led to an almost two-fold increase of the molecular weight, according to size exclusion (SEC) multiangle laser light scattering (MALLS) analysis. In contrast, SrLA showed considerably less activity on lignosulfonates, as measured based on oxygen consumption and SEC-MALLS.
AB - Lignin is the second most abundant biopolymer on earth and accrues in large amounts in the pulp- and paper industry. While currently lignins are mainly used for low-value applications such as energy production, recently the potential of laccases for upgrading lignins has been demonstrated. In this study, two laccases of fungal and bacterial origin were characterized regarding their potential to polymerize lignosulfonate. The laccases MaL1 from Melanocarpus albomyces, and SrLA from Streptomyces rochei were heterologously expressed and showed typical characteristics of laccases, like acidic pH optima for ABTS at pH 4 and 5, respectively, and temperature optima at 50 °C and 80 °C. Polymerization of lignosulfonate with MaL1 led to an almost two-fold increase of the molecular weight, according to size exclusion (SEC) multiangle laser light scattering (MALLS) analysis. In contrast, SrLA showed considerably less activity on lignosulfonates, as measured based on oxygen consumption and SEC-MALLS.
KW - Laccase
KW - Lignin
KW - Lignosulfonate polymerization
UR - http://www.scopus.com/inward/record.url?scp=85111027362&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2021.07.001
DO - 10.1016/j.procbio.2021.07.001
M3 - Article
SN - 1359-5113
VL - 109
SP - 207
EP - 213
JO - Process Biochemistry
JF - Process Biochemistry
ER -