Current advances in engineering tools for Pichia pastoris

Jasmin E. Fischer; Anton Glieder

doi:10.1016/j.copbio.2019.06.002

Current advances in engineering tools for Pichia pastoris

Jasmin E. Fischer, Anton Glieder

Institute of Molecular Biotechnology (6550)

Research output: Contribution to journal › Review article › peer-review

Abstract

In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	Current Opinion in Biotechnology
Volume	59
DOIs	https://doi.org/10.1016/j.copbio.2019.06.002
Publication status	Published - 1 Oct 2019

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

Access to Document

10.1016/j.copbio.2019.06.002

Cite this

@article{c0b6894dd4274a0e864f51d4007c6b9e,

title = "Current advances in engineering tools for Pichia pastoris",

abstract = "In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.",

author = "Fischer, {Jasmin E.} and Anton Glieder",

year = "2019",

month = oct,

day = "1",

doi = "10.1016/j.copbio.2019.06.002",

language = "English",

volume = "59",

pages = "175--181",

journal = "Current Opinion in Biotechnology",

issn = "0958-1669",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Current advances in engineering tools for Pichia pastoris

AU - Fischer, Jasmin E.

AU - Glieder, Anton

PY - 2019/10/1

Y1 - 2019/10/1

N2 - In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

AB - In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

UR - http://www.scopus.com/inward/record.url?scp=85071239727&partnerID=8YFLogxK

U2 - 10.1016/j.copbio.2019.06.002

DO - 10.1016/j.copbio.2019.06.002

M3 - Review article

C2 - 31470258

AN - SCOPUS:85071239727

SN - 0958-1669

VL - 59

SP - 175

EP - 181

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

ER -

Current advances in engineering tools for Pichia pastoris

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this