Development of a molecular approach to describe the composition of Trichoderma communities

R. Meincke, N. Weinert, V. Radl, M. Schloter, K. Smalla, Gabriele Berg

Research output: Contribution to journalArticlepeer-review


Trichoderma and its teleomorphic stage Hypocrea play a key role for ecosystem functioning in terrestrial habitats. However, little is known about the ecology of the fungus. In this study we developed a novel Trichoderma-specific primer pair for diversity analysis. Based on a broad range master alignment, specific Trichoderma primers (ITSTrF/ITSTrR) were designed that comprise an approximate 650 bp fragment of the internal transcribed spacer region from all taxonomic clades of the genus Trichoderma. This amplicon is suitable for identification with TrichoKey and TrichoBLAST. Moreover, this primer system was successfully applied to study the Trichoderma communities in the rhizosphere of different potato genotypes grown at two field sites in Germany. Cloning and sequencing confirmed the specificity of the primer and revealed a site-dependent Trichoderma composition. Based on the new primer system a semi …
Original languageEnglish
Pages (from-to)63-69
JournalJournal of microbiological methods
Publication statusPublished - 2009

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)


Dive into the research topics of 'Development of a molecular approach to describe the composition of Trichoderma communities'. Together they form a unique fingerprint.

Cite this