Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

Birgit Wiltschi; Tomislav Cernava; Alexander Dennig; Meritxell Galindo; Martina Geier; Steffen Gruber; Marianne Haberbauer; Petra Heidinger; Enrique Herrero Acero; Regina Kratzer; Christiane Luley-Goedl; Christina A Müller; Julia Pitzer; Doris Ribitsch; Michael Sauer; Katharina Schmölzer; Wolfgang Schnitzhofer; Christoph W Sensen; Jung Soh; Kerstin Steiner; Christoph K Winkler; Margit Winkler; Tamara Wriessnegger

doi:10.1016/j.biotechadv.2020.107520

Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

Birgit Wiltschi^*, Tomislav Cernava, Alexander Dennig, Meritxell Galindo, Martina Geier, Steffen Gruber, Marianne Haberbauer, Petra Heidinger, Enrique Herrero Acero, Regina Kratzer, Christiane Luley-Goedl, Christina A Müller, Julia Pitzer, Doris Ribitsch, Michael Sauer, Katharina Schmölzer, Wolfgang Schnitzhofer, Christoph W Sensen, Jung Soh, Kerstin SteinerChristoph K Winkler, Margit Winkler, Tamara Wriessnegger

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

Abstract

Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO2 into valuable products and biocontrol in agriculture, to recycling of synthetic polymers and recovery of precious metals.

Original language	English
Article number	107520
Journal	Biotechnology Advances
Volume	40
DOIs	https://doi.org/10.1016/j.biotechadv.2020.107520
Publication status	Published - 23 Jan 2020

Keywords

Biocatalysis
Combinatorial DNA assembly
Downstream processing
Enzyme cascade
Enzyme discovery
Enzyme engineering
Gene model
Host strain design
Process design
Whole-cell biotransformation

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology (miscellaneous)
Biotechnology
Applied Microbiology and Biotechnology
Bioengineering

Fields of Expertise

Human- & Biotechnology

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Wiltschi, B., Cernava, T., Dennig, A., Galindo, M., Geier, M., Gruber, S., Haberbauer, M., Heidinger, P., Acero, E. H., Kratzer, R., Luley-Goedl, C., Müller, C. A., Pitzer, J., Ribitsch, D., Sauer, M., Schmölzer, K., Schnitzhofer, W., Sensen, C. W., Soh, J., ... Wriessnegger, T. (2020). Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications. Biotechnology Advances, 40, Article 107520. https://doi.org/10.1016/j.biotechadv.2020.107520

Wiltschi, B, Cernava, T, Dennig, A, Galindo, M, Geier, M, Gruber, S, Haberbauer, M, Heidinger, P, Acero, EH, Kratzer, R, Luley-Goedl, C, Müller, CA, Pitzer, J, Ribitsch, D, Sauer, M, Schmölzer, K, Schnitzhofer, W, Sensen, CW, Soh, J, Steiner, K, Winkler, CK, Winkler, M & Wriessnegger, T 2020, 'Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications', Biotechnology Advances, vol. 40, 107520. https://doi.org/10.1016/j.biotechadv.2020.107520

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abstract = "Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO2 into valuable products and biocontrol in agriculture, to recycling of synthetic polymers and recovery of precious metals.",

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author = "Birgit Wiltschi and Tomislav Cernava and Alexander Dennig and Meritxell Galindo and Martina Geier and Steffen Gruber and Marianne Haberbauer and Petra Heidinger and Acero, {Enrique Herrero} and Regina Kratzer and Christiane Luley-Goedl and M{\"u}ller, {Christina A} and Julia Pitzer and Doris Ribitsch and Michael Sauer and Katharina Schm{\"o}lzer and Wolfgang Schnitzhofer and Sensen, {Christoph W} and Jung Soh and Kerstin Steiner and Winkler, {Christoph K} and Margit Winkler and Tamara Wriessnegger",

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doi = "10.1016/j.biotechadv.2020.107520",

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AU - Wiltschi, Birgit

AU - Cernava, Tomislav

AU - Dennig, Alexander

AU - Galindo, Meritxell

AU - Geier, Martina

AU - Gruber, Steffen

AU - Haberbauer, Marianne

AU - Heidinger, Petra

AU - Acero, Enrique Herrero

AU - Kratzer, Regina

AU - Luley-Goedl, Christiane

AU - Müller, Christina A

AU - Pitzer, Julia

AU - Ribitsch, Doris

AU - Sauer, Michael

AU - Schmölzer, Katharina

AU - Schnitzhofer, Wolfgang

AU - Sensen, Christoph W

AU - Soh, Jung

AU - Steiner, Kerstin

AU - Winkler, Christoph K

AU - Winkler, Margit

AU - Wriessnegger, Tamara

PY - 2020/1/23

Y1 - 2020/1/23

N2 - Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO2 into valuable products and biocontrol in agriculture, to recycling of synthetic polymers and recovery of precious metals.

AB - Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO2 into valuable products and biocontrol in agriculture, to recycling of synthetic polymers and recovery of precious metals.

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KW - Combinatorial DNA assembly

KW - Downstream processing

KW - Enzyme cascade

KW - Enzyme discovery

KW - Enzyme engineering

KW - Gene model

KW - Host strain design

KW - Process design

KW - Whole-cell biotransformation

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VL - 40

JO - Biotechnology Advances

JF - Biotechnology Advances

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ER -

Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

Abstract

Keywords

ASJC Scopus subject areas

Fields of Expertise

Treatment code (Nähere Zuordnung)

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