Abstract
A global analysis model has been developed for randomly oriented, fully hydrated, inverted hexagonal (H II ) phases formed by many amphiphiles in aqueous solution, including membrane lipids. The model is based on a structure factor for hexagonally packed rods and a compositional model for the scattering length density, enabling also the analysis of positionally weakly correlated H II phases. Bayesian probability theory was used for optimization of the adjustable parameters, which allows parameter correlations to be retrieved in much more detail than standard analysis techniques and thereby enables a realistic error analysis. The model was applied to different phosphatidylethanolamines, including previously unreported H II data for diC14:0 and diC16:1 phosphatidylethanolamine. The extracted structural features include intrinsic lipid curvature, hydrocarbon chain length and area per lipid at the position of the neutral plane.
Original language | English |
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Pages (from-to) | 403-414 |
Number of pages | 12 |
Journal | Journal of Applied Crystallography |
Volume | 52 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Apr 2019 |
Keywords
- Bayesian analysis
- full-q-range analysis
- intrinsic curvature
- inverted hexagonal phases
- phospholipids
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology