Abstract
Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.
Original language | English |
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Pages (from-to) | 24918-24945 |
Number of pages | 28 |
Journal | International Journal of Molecular Sciences |
Volume | 16 |
Issue number | 10 |
DOIs | |
Publication status | Published - 20 Oct 2015 |
Externally published | Yes |
Keywords
- FACS-based screening platforms
- High-throughput screening
- In vitro compartmentalization
- Microfluidics-based screening platforms
- Protein engineering
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Computer Science Applications
- Spectroscopy
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry