Abstract
The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor‐recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole‐cell biotransformations. This increases atom efficiency and simplifies the reaction. We have expressed a recombinant ene‐reductase in the hydrogen‐oxidizing proteobacterium Cupriavidus necator H16. In hydrogen‐driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99% ee. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive as it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.
Original language | English |
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Pages (from-to) | 2361-2365 |
Number of pages | 5 |
Journal | ChemSusChem |
Volume | 12 |
Issue number | 11 |
Early online date | 19 Mar 2019 |
DOIs | |
Publication status | Published - 19 Mar 2019 |
Keywords
- Hydrogenation
- Biotransformations
- Biocatalysis
- Assymetric synthesis
- Reduction
- biotransformation
- asymmetric synthesis
- reduction
- hydrogenation
- biocatalysis
ASJC Scopus subject areas
- Energy(all)
- Chemical Engineering(all)
- Materials Science(all)
- Environmental Chemistry
Fields of Expertise
- Human- & Biotechnology