Verticillium wilt is an important disease in oilseed rape with an increasing importance worldwide. Currently, there are no methods available to suppress the pathogen. A biological protection strategy on the basis of the plant-beneficial bacterium Serratia plymuthica HRO-C48 to control Verticillium dahliae in oilseed rape was developed. Three different techniques to apply the biocontrol agent to seeds, namely pelleting, film coating and bio-priming, were evaluated considering the influence on the control activity, cell stability during storage and practical feasibility. Neither the treatment nor the inoculum density was found to influence the abundances of HRO-C48 in the rhizosphere after 30 days. Serratia treatment using bio-priming and pelleting resulted in a statistically significant biocontrol in comparison to the non-bacterized controls. Additionally, survival of HRO-C48 differed between treatments, and was the highest using bio-priming at 20°C, and pelleting at 4°C. In conclusion, the procedure of bio-priming, which was developed in line with this study, resulted in a stable and efficient formulation of S. plymuthica on rape seed. This technology opens a possibility to develop a commercial Serratia formulation to protect oilseed against V. dahliae.