Impact of growth factors on wound healing in polysaccharide blend thin films

Uroš Maver, Lidija Gradišnik, Dragica Maja Smrke, Karin Stana Kleinschek*, Tina Maver

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The potential of alginate/carboxymethyl cellulose based thin films was demonstrated as a testing platform for evaluation of the influence of in situ incorporated growth factors into formulations intended for 3D bio-printed wound healing materials. Actual formation of blend films with and without included growth factors was analysed using ATR-IR spectroscopy. Surface morphology and topography were evaluated by atomic force and scanning electron microscopies, whereas the hydrophilicity of all tested materials was determined using the water contact angle measurement method. The inclusion of growth factors into blend films did not affect the films' formation, nor their morphology, whereas their highly hydrophilic character was even slightly enhanced by the added growth factors. Further, several aspects (viability, proliferation and cell morphology), in regard to the blend films included growth factors' influence on both of the most abundant skin cell types (keratynocytes and fibroblasts) were tested. A significantly improved cell viability was detected for films with incorporated growth factors. The prepared thin film-based testing platform could present an important tool to aid the development of novel printable (bio)inks.

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalApplied Surface Science
Publication statusPublished - 30 Sept 2019
Externally publishedYes


  • Alginate
  • Carboxymethyl cellulose
  • Growth factors
  • Thin films
  • Wound healing

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films


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