Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanová; Astrid Weninger; Anton Glieder; Karin Kovar; Thomas Vogl

doi:10.1016/j.biotechadv.2018.01.006

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanová, Astrid Weninger, Anton Glieder, Karin Kovar, Thomas Vogl^*

^*Corresponding author for this work

Institute of Molecular Biotechnology (6550)

Research output: Contribution to journal › Review article › peer-review

Abstract

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

Original language	English
Pages (from-to)	641-665
Number of pages	25
Journal	Biotechnology Advances
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/j.biotechadv.2018.01.006
Publication status	Published - 1 May 2018

Keywords

Candida albicans/glabrata
CRISPR-Cas
Expression optimization
Metabolic engineering
Pichia pastoris
Saccharomyces cerevisiae
Schizosaccharomyces pombe
Synthetic biology
Yarrowia lipolytica
Yeasts

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects",

abstract = "Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.",

keywords = "Candida albicans/glabrata, CRISPR-Cas, Expression optimization, Metabolic engineering, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Synthetic biology, Yarrowia lipolytica, Yeasts",

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year = "2018",

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doi = "10.1016/j.biotechadv.2018.01.006",

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T2 - Current state and future prospects

AU - Raschmanová, Hana

AU - Weninger, Astrid

AU - Glieder, Anton

AU - Kovar, Karin

AU - Vogl, Thomas

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

AB - Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

KW - Candida albicans/glabrata

KW - CRISPR-Cas

KW - Expression optimization

KW - Metabolic engineering

KW - Pichia pastoris

KW - Saccharomyces cerevisiae

KW - Schizosaccharomyces pombe

KW - Synthetic biology

KW - Yarrowia lipolytica

KW - Yeasts

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SN - 0734-9750

VL - 36

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JO - Biotechnology Advances

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ER -

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Abstract

Keywords

ASJC Scopus subject areas

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