In-Vial Printing and Drying of Biologics as a Personalizable Approach

Daniela Fiedler, Carolina Alva , Joana Pinto, Martin Spörk, Ramona Jeitler, Eva Roblegg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


This study addressed the need for a flexible (personalizable) production of biologics, allowing their stabilization in the solid state and processing of small batch volumes. Therefore, inkjet printing into vials followed by a gentle vacuum drying step at ambient temperature was investigated by screening different formulations with a 2 2-full factorial design of experiments regarding printability. Human Serum Albumin (HSA) was used as a model protein in a wide range of concentrations (5 to 50 mg/ml), with (10 w/v%) and without the surfactant polysorbate 80 (PS80). PS80 was identified to positively affect the formulations by increasing the Ohnesorge number and stabilizing the printing process. The dispensed volumes with a target dose of 0.5 mg HSA were dried and analyzed concerning their residual moisture (RM) and protein aggregation. All investigated formulations showed an RM < 10 wt.% and no significant induced protein aggregation as confirmed by Size Exclusion Chromatography (< 2.5%) and Dynamic Light Scattering (Aggregation Index ≤ 2.5). Additionally, long-term printability and the available final dose after reconstitution were investigated for two optimized formulations. A promising formulation providing ∼93% of the targeted dose and a reconstitution time of 30s was identified.
Original languageEnglish
Article number121909
JournalInternational Journal of Pharmaceutics
Publication statusPublished - 25 Jul 2022


  • inkjet printing
  • personalized manufacturing
  • polysorbate 80
  • drying proteins
  • human serum albumin
  • Human serum albumin
  • Drying proteins
  • Inkjet printing
  • Personalized manufacturing
  • Polysorbate 80

ASJC Scopus subject areas

  • Pharmaceutical Science


  • BioTechMed-Graz


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