LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang; Marco Vilela; Andreas Winkler; Miroslaw Tarnawski; Ilme Schlichting; Hayretin Yumerefendi; Brian Kuhlman; Rihe Liu; Gaudenz Danuser; Klaus M Hahn

doi:10.1038/nmeth.3926

LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M Hahn

Institute of Biochemistry (6480)

Research output: Contribution to journal › Article › peer-review

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original language	English
Pages (from-to)	755-758
Journal	Nature Methods
Volume	13
DOIs	https://doi.org/10.1038/nmeth.3926
Publication status	Published - 2016

Keywords

optogenetics
cell signalling
cell migration

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1038/nmeth.3926

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title = "LOVTRAP: an optogenetic system for photoinduced protein dissociation",

abstract = "LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.",

keywords = "optogenetics, cell signalling, cell migration",

author = "Hui Wang and Marco Vilela and Andreas Winkler and Miroslaw Tarnawski and Ilme Schlichting and Hayretin Yumerefendi and Brian Kuhlman and Rihe Liu and Gaudenz Danuser and Hahn, {Klaus M}",

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T1 - LOVTRAP: an optogenetic system for photoinduced protein dissociation

AU - Wang, Hui

AU - Vilela, Marco

AU - Winkler, Andreas

AU - Tarnawski, Miroslaw

AU - Schlichting, Ilme

AU - Yumerefendi, Hayretin

AU - Kuhlman, Brian

AU - Liu, Rihe

AU - Danuser, Gaudenz

AU - Hahn, Klaus M

PY - 2016

Y1 - 2016

N2 - LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

AB - LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

KW - optogenetics

KW - cell signalling

KW - cell migration

U2 - 10.1038/nmeth.3926

DO - 10.1038/nmeth.3926

M3 - Article

SN - 1548-7105

VL - 13

SP - 755

EP - 758

JO - Nature Methods

JF - Nature Methods

ER -

LOVTRAP: an optogenetic system for photoinduced protein dissociation

Abstract

Keywords

ASJC Scopus subject areas

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