Abstract
To date, efficiency upon non-viral DNA delivery remains low and this implies the existence of unidentified transfection barriers. Here we explore the mechanisms of action of multicomponent (MC) cationic liposome/DNA complexes (lipoplexes) by a combination of reporter technologies, dynamic light scattering (DLS), synchrotron small angle X-ray scattering (SAXS), fluorescence activated cell sorting (FACS) analysis and laser scanning confocal microscopy (LSCM) in live cells. Lipofectamine – the gold standard among transfection reagents – was used as a reference. On the basis of our results, we suggest that an additional transfection barrier impairs transfection efficiency, that is: low lipoplex concentration at the cell surface. Based on the acquired knowledge we propose an optimized transfection protocol that allowed us to efficiently transfect DND41, JURKAT, MOLT3, P12-ICHIKAWA, ALL-SILL, TALL-1 human T-cell acute lymphoblastic leukemia (T-ALL) cell lines known to be difficult-to-transfect by using non-viral vectors and where LFN-based technologies fail to give satisfactory results.
Original language | English |
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Pages (from-to) | 681-691 |
Number of pages | 11 |
Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
Volume | 13 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Feb 2017 |
Keywords
- Cationic liposomes
- Cell transfection
- Hard-to-transfect cells
- Lipoplexes
- Transfection efficiency
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Bioengineering
- Biomedical Engineering
- Molecular Medicine
- Materials Science(all)
- Pharmaceutical Science
Fields of Expertise
- Advanced Materials Science
- Human- & Biotechnology