Non-coding Natural Antisense Transcripts: Analysis and Application

Julian C. Krappinger, Lilli Bonstingl, Katrin Pansy, Katja Sallinger, Nick I. Wreglesworth, Lukas Grinninger, Alexander Deutsch, Amin El-Heliebi, Thomas Kroneis, Ramsay J. Mcfarlane, Christoph W. Sensen, Julia Feichtinger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Non-coding natural antisense transcripts (ncNATs) are regulatory RNA molecules that are overlapping with as well as complementary to other transcripts. These transcripts are implicated in a broad variety of biological and pathological processes, including tumorigenesis and oncogenic progression. With this complex field still in its infancy, annotations, expression profiling and functional characterisations of ncNATs are far less comprehensive than those for protein-coding genes, pointing out substantial gaps in the analysis and characterisation of these regulatory transcripts. In this review, we discuss ncNATs from an analysis perspective, in particular regarding the use of high-throughput sequencing strategies, such as RNA-sequencing, and summarize the unique challenges of investigating the antisense transcriptome. Finally, we elaborate on their potential as biomarkers and future targets for treatment, focusing on cancer.

Original languageEnglish
Pages (from-to)75-101
Number of pages27
JournalJournal of Biotechnology
Publication statusPublished - 10 Nov 2021


  • biomarkers
  • liquid biopsy
  • long non-coding RNAs
  • natural antisense transcripts
  • strand-specific RNA-seq

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Non-coding Natural Antisense Transcripts: Analysis and Application'. Together they form a unique fingerprint.

Cite this