Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste

Alexander Muhr, Eva Maria Rechberger, Anna Salerno, Angelika Reiterer, Karin Malli, Katharina Strohmeier, Sigurd Schober, Martin Mittelbach, Martin Koller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (μmax.) of 0.08 h−1, 0.10 h−1 and 0.13 h−1, respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum.
Original languageEnglish
Pages (from-to)45-51
JournalJournal of Biotechnology
Volume165
Issue number1
DOIs
Publication statusPublished - 2013

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)
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  • Application
  • Experimental

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