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Abstract
A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (μmax.) of 0.08 h−1, 0.10 h−1 and 0.13 h−1, respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum.
Original language | English |
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Pages (from-to) | 45-51 |
Journal | Journal of Biotechnology |
Volume | 165 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2013 |
Fields of Expertise
- Human- & Biotechnology
Treatment code (Nähere Zuordnung)
- Basic - Fundamental (Grundlagenforschung)
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- Application
- Experimental
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Dive into the research topics of 'Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste'. Together they form a unique fingerprint.Projects
- 1 Finished
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EU - ANIMPOL - Biotechnological conversion of carbon containing wastes for eco-efficient production of high added value products
Narodoslawsky, M., Koller, M., Schnitzer, H. & Nidetzky, B.
1/01/10 → 31/12/12
Project: Research project