Since the first successful directed evolution studies in the 1990s, experience shows that the success of protein engineering for an enzyme of interest mainly depends on the extent of knowledge of its structure-function relationships and the ability to characterize a sufficiently large number of enzyme variants.1 A good understanding of a catalytic property makes it possible to achieve an objective with very few amino acid exchanges. In contrast, a powerful assay makes it possible to extend the number of investigated variants and thus to increase the likelihood to find an improved mutant.
|Title of host publication||Protein Engineering- Methods and Protocols|
|Editors||Uwe Bornscheuer, Matthias Höhne|
|Number of pages||18|
|Publication status||Published - 1 Sept 2017|
|Name||Springer Protocols Methods in Molecular Biology|