Practical Considerions Regarding the Choice of High-Throughput-Assays

Robert Kourist, Carolin Mügge

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Since the first successful directed evolution studies in the 1990s, experience shows that the success of protein engineering for an enzyme of interest mainly depends on the extent of knowledge of its structure-function relationships and the ability to characterize a sufficiently large number of enzyme variants.1 A good understanding of a catalytic property makes it possible to achieve an objective with very few amino acid exchanges. In contrast, a powerful assay makes it possible to extend the number of investigated variants and thus to increase the likelihood to find an improved mutant.
Original languageEnglish
Title of host publicationProtein Engineering- Methods and Protocols
EditorsUwe Bornscheuer, Matthias Höhne
Number of pages18
Publication statusPublished - 1 Sept 2017

Publication series

NameSpringer Protocols Methods in Molecular Biology
PublisherHumana Press

Fields of Expertise

  • Human- & Biotechnology

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