Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments

Christine Moissl-Eichinger, Anna K. Auerbach, Alexander J. Probst, Alexander Mahnert, Tom Lauren, Yvette Piceno, Gary L. Andersen, Kasthuri Venkateswaran, Petra Rettberg, Simon Barczyk, Rüdiger Pukall, Gabriele Berg

Research output: Contribution to journalArticlepeer-review


Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony …
Original languageEnglish
Pages (from-to)1-13
JournalScientific Reports
Issue number9156
Publication statusPublished - 2015

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

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