Regiospecific 7-hydroxylation of ten-carbon monoterpenes by detoxifying CYP5035S7 monooxygenase of the white-rot fungus Polyporus arcularius

Nico D Fessner*, Hansjörg Weber, Anton Glieder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In a previous study, we identified CYP5035S7 of the white-rot fungus Polyporus arcularius with a broad activity towards monoterpenes such as p-cymene. Therefore, in this study we aimed at further exploring the substrate scope of detoxifying CYP5035S7 towards terpenes and semi-preparatively isolating some of the products via whole-cell biotransformation, in order to obtain information about the enzyme's reactivity. We noticed a clear preference for the monoterpene skeleton and elucidated a distinct regioselectivity pattern based on key structural and electronic features of its substrates. This study illustrates how minimal characterisation effort may already suffice to provide vital information on enzymatic reactivity by the comparison of structural derivatives.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalBiochemical and Biophysical Research Communications
Publication statusPublished - 5 Mar 2022


  • biocatalysis


  • NAWI Graz

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