Abstract
Cancer cells typically exhibit altered ion channel expression and membrane potential, which plays an important role in various cellular processes such as proliferation or apoptosis. Hence, therapeutic strategies that modulate ion channel activity or shift the membrane potential prove to be promising for cancer therapy. Since temperature significantly affects ion channel function, deviations from normal body temperature might provide a key factor in electrophysiological processes, potentially affecting cell proliferation and thus tumor progression. In this pilot study, we focused on a first insight into the temperature-induced modulation of A549 cells using an automated patch clamp system. Measurements of 14 cells at hypo-, normo- and hyperthermia were accomplished to investigate the temperature-dependent electrophysiological behavior. The results revealed that both, temperatures below and above normal body temperature affect the current and potential of A549 cells. In hyperthermia the potential tends to more hyperpolarized values, while conversely, in hypothermic conditions we observed mainly a lower current response and thus a depolarization of the membrane potential.
Original language | English |
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Pages (from-to) | 85-88 |
Number of pages | 4 |
Journal | Current Directions in Biomedical Engineering |
Volume | 8 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Aug 2022 |
Keywords
- ion channel kinetics
- membrane potential
- oncological hyperthermia
- temperature modulation
ASJC Scopus subject areas
- Biomedical Engineering
Fields of Expertise
- Human- & Biotechnology