TY - JOUR
T1 - Thermo-responsive lipophilic NIPAM-based block copolymers as stabilizers for lipid-based cubic nanoparticles
AU - Balestri, Arianna
AU - Lonetti, Barbara
AU - Harrisson, Simon
AU - Farias-Mancilla, Barbara
AU - Zhang, Junliang
AU - Amenitsch, Heinz
AU - Schubert, Ulrich S.
AU - Guerrero-Sanchez, Carlos
AU - Montis, Costanza
AU - Berti, Debora
N1 - Funding Information:
Funding: This work was supported by MIUR -Italy (“Progetto Dipartimenti di Eccellenza 2018–2022, allocated to Department of Chemistry ”Ugo Schiff”). The authors acknowledge the CERIC-ERIC Consortium for the access to experimental facilities and the beam time. The authors thank the technical support in Cryo-TEM measurements performed in the FloCEN Florence Center for Electron Nanoscopy of the University of Florence. All the authors acknowledge Pietro Grazi for his contribution on DLS measurements of cubosomes stabilized by F127. S.H, B.F.M. and B.L. acknowledge the Agence Nationale de la Recherche ( ANR , France, ANR project: ANR-15-CE08-0039 ).U.S.S. and C.G.-S. thank the financial support of the Deutsche Forschungsgemeinschaft (DFG, Germany) for funding the Collaborative Research Center PolyTarget (SFB 1278—project number 316213987, projects A01, B02, and Z01).
Funding Information:
Funding: This work was supported by MIUR-Italy (“Progetto Dipartimenti di Eccellenza 2018–2022, allocated to Department of Chemistry ”Ugo Schiff”). The authors acknowledge the CERIC-ERIC Consortium for the access to experimental facilities and the beam time. The authors thank the technical support in Cryo-TEM measurements performed in the FloCEN Florence Center for Electron Nanoscopy of the University of Florence. All the authors acknowledge Pietro Grazi for his contribution on DLS measurements of cubosomes stabilized by F127. S.H, B.F.M. and B.L. acknowledge the Agence Nationale de la Recherche (ANR, France, ANR project: ANR-15-CE08-0039).U.S.S. and C.G.-S. thank the financial support of the Deutsche Forschungsgemeinschaft (DFG, Germany) for funding the Collaborative Research Center PolyTarget (SFB 1278—project number 316213987, projects A01, B02, and Z01).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/12
Y1 - 2022/12
N2 - The design of drug delivery systems (DDS) for the encapsulation of therapeutic agents and the controlled release to the target site of the disease is one of the main goals of nanomedicine. Although already explored in an extensive number of studies over the years, lipid assemblies, and particularly liposomes, are still considered the most promising and interesting candidates as DDS due to their biocompatibility and structural similarity with plasma membranes. Lately, this research area has been extended to include more complex lipid assemblies, such as cubosomes. Cubosomes are an emerging structural platform for the delivery of molecules with pharmaceutical interest, such as drugs, bioactives and contrast agents. Here we report on the application of a thermo-responsive copolymer poly(N,N-dimethylacrylamide)-block-poly(N-isopropylacrylamide) (PDMA-b-PNIPAM), as a thermoresponsive stabilizer of lipid-based nanoparticles for drug-delivery. First, we assessed the affinity of PDMA-b-PNIPAM towards supported and free-standing bilayers; then, we explored the colloidal and thermoresponsive properties of cubic self-assembled DDS composed of glycerol-monooleate (GMO), where PDMA-b-PNIPAM replaces the conventional stabilizer Pluronic F127 (PEOx-PPOy-PEOx), normally used for cubosomes. We prepared dispersions of cubic lipid nanoparticles with two PDMA-b-PNIPAM block copolymers of different molar mass. The colloidal properties were then assessed and compared to those exhibited by standard lipid cubic dispersions stabilized by Pluronic F-127, combining a series of experimental techniques (Quartz Crystal Microbalance with Dissipation monitoring, Dynamic Light Scattering, Small-Angle X-rays Scattering, Cryo-Transmission Electron Microscopy). Interestingly, PDMA-b-PNIPAM stabilized cubosomes display additional benefits with respect to those stabilized by Pluronic, thanks to the combination of a “sponge “ effect for the controlled release of encapsulated molecules and an increased affinity towards lipid bilayer membranes, which is a promising feature to maximize fusion with the target-cellular site.
AB - The design of drug delivery systems (DDS) for the encapsulation of therapeutic agents and the controlled release to the target site of the disease is one of the main goals of nanomedicine. Although already explored in an extensive number of studies over the years, lipid assemblies, and particularly liposomes, are still considered the most promising and interesting candidates as DDS due to their biocompatibility and structural similarity with plasma membranes. Lately, this research area has been extended to include more complex lipid assemblies, such as cubosomes. Cubosomes are an emerging structural platform for the delivery of molecules with pharmaceutical interest, such as drugs, bioactives and contrast agents. Here we report on the application of a thermo-responsive copolymer poly(N,N-dimethylacrylamide)-block-poly(N-isopropylacrylamide) (PDMA-b-PNIPAM), as a thermoresponsive stabilizer of lipid-based nanoparticles for drug-delivery. First, we assessed the affinity of PDMA-b-PNIPAM towards supported and free-standing bilayers; then, we explored the colloidal and thermoresponsive properties of cubic self-assembled DDS composed of glycerol-monooleate (GMO), where PDMA-b-PNIPAM replaces the conventional stabilizer Pluronic F127 (PEOx-PPOy-PEOx), normally used for cubosomes. We prepared dispersions of cubic lipid nanoparticles with two PDMA-b-PNIPAM block copolymers of different molar mass. The colloidal properties were then assessed and compared to those exhibited by standard lipid cubic dispersions stabilized by Pluronic F-127, combining a series of experimental techniques (Quartz Crystal Microbalance with Dissipation monitoring, Dynamic Light Scattering, Small-Angle X-rays Scattering, Cryo-Transmission Electron Microscopy). Interestingly, PDMA-b-PNIPAM stabilized cubosomes display additional benefits with respect to those stabilized by Pluronic, thanks to the combination of a “sponge “ effect for the controlled release of encapsulated molecules and an increased affinity towards lipid bilayer membranes, which is a promising feature to maximize fusion with the target-cellular site.
KW - Amphiphilic block copolymer
KW - Cubosomes
KW - Drug delivery
KW - Model membrane
KW - Stabilizer
UR - http://www.scopus.com/inward/record.url?scp=85139294974&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2022.112884
DO - 10.1016/j.colsurfb.2022.112884
M3 - Article
C2 - 36209550
AN - SCOPUS:85139294974
VL - 220
JO - Colloids and Surfaces / B
JF - Colloids and Surfaces / B
SN - 0927-7765
M1 - 112884
ER -