Whole cell carboxylate reduction for the synthesis of 3-hydroxytyrosol

Kamila Napora, Karen Robins, Antonio Osorio-Lozada, Margit Winkler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

3-Hydroxytyrosol (3-HT) is a phenolic antioxidant that has a number of beneficial effects on human health and is a valuable building block in the synthesis of various pharmaceuticals. Herein, we report a new method for the production of 3-HT through reduction of 3,4-dihydroxyphenylacetic acid. The reduction was performed in whole Escherichia coli BL21 (DE3) cells overexpressing carboxylic acid reductase from Nocardia and phosphopantetheinyl transferase from E. coli. An endogenous E. coli aldehyde reducing activity turned out to be highly efficient for further reduction of the aldehyde intermediate to the desired alcohol. The influence of different buffer components, cofactors, and cofactor recycling systems was investigated. A very economic combination of glucose, citrate, and air proved sufficient for recycling of the essential cofactors ATP and NAD(P)H. Selected crucial parameters were then further optimized within a “design of experiments” approach. Finally, first preparative-scale bioreductions resulted in pure 3-HT.
Original languageEnglish
Pages (from-to)1089-1095
JournalChemCatChem
Volume6
Issue number4
DOIs
Publication statusPublished - 2014

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Application
  • Experimental

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