TY - JOUR
T1 - Characterization and optimization of a quantitative colorimetric acetylcholine esterase inhibition assay for biochip integration demonstrated by neurotoxicity evaluation of malathion
AU - Vasconez Martinez, Mateo G.
AU - Parato, Noemi
AU - Schobesberger, Silvia
AU - Selinger, Florian
AU - Reihs, Eva I.
AU - Spitz, Sarah
AU - Frauenlob, Martin
AU - Ertl, Peter
AU - Resch, Christian
AU - Bauer, Gerald
AU - Povoden, Günter
AU - Rothbauer, Mario
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/6
Y1 - 2024/6
N2 - Organophosphates (OPs) and carbamates as insecticides, nematicides, fungicides, and herbicides are constantly increasing. Their neurotoxic nature requires careful usage, and misuse can lead to fatalities. OPs classified as 'Class 1′ toxic compounds irreversibly inhibit cholinesterases due to their molecular structure resembling the natural acetylcholine substrate, leading to toxic events in the human brain. Monitoring such chemicals is relevant for agricultural applications and essential for the military sector to ensure the safety of personnel and civilian populations. State-of-the-art analytical detection methods require time-consuming pre-treatments and costly reagents and face challenges associated with pesticide properties like thermal lability, low volatility, and high polarity, which can compromise analysis performance. Advanced systems like electrophoresis or liquid chromatography are used to address these, but these are not well suited for field analysis. Miniaturized colorimetric assays are becoming more popular for various portable devices and kits (i.e., metabolic or blood cell assays) due to their ease of use and practicality. Here, we aimed to establish and optimize a straight-forward paper-based microfluidic acetylcholine esterase inhibition assay for mobile organophosphate detection, laying the groundwork for future microdevice modules to be used in environmental monitoring, public health, and CBRN applications.
AB - Organophosphates (OPs) and carbamates as insecticides, nematicides, fungicides, and herbicides are constantly increasing. Their neurotoxic nature requires careful usage, and misuse can lead to fatalities. OPs classified as 'Class 1′ toxic compounds irreversibly inhibit cholinesterases due to their molecular structure resembling the natural acetylcholine substrate, leading to toxic events in the human brain. Monitoring such chemicals is relevant for agricultural applications and essential for the military sector to ensure the safety of personnel and civilian populations. State-of-the-art analytical detection methods require time-consuming pre-treatments and costly reagents and face challenges associated with pesticide properties like thermal lability, low volatility, and high polarity, which can compromise analysis performance. Advanced systems like electrophoresis or liquid chromatography are used to address these, but these are not well suited for field analysis. Miniaturized colorimetric assays are becoming more popular for various portable devices and kits (i.e., metabolic or blood cell assays) due to their ease of use and practicality. Here, we aimed to establish and optimize a straight-forward paper-based microfluidic acetylcholine esterase inhibition assay for mobile organophosphate detection, laying the groundwork for future microdevice modules to be used in environmental monitoring, public health, and CBRN applications.
KW - AChE
KW - Colorimetry
KW - Enzyme inhibition assay
KW - Malathion
KW - Microfluidics
KW - Neurotoxicity
KW - Organophosphates
UR - http://www.scopus.com/inward/record.url?scp=85186536577&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2024.135568
DO - 10.1016/j.snb.2024.135568
M3 - Article
AN - SCOPUS:85186536577
SN - 0925-4005
VL - 408
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 135568
ER -