TY - JOUR
T1 - Deep tissue localization and sensing using optical microcavity probes
AU - Kavčič, Aljaž
AU - Garvas, Maja
AU - Marinčič, Matevž
AU - Unger, Katrin
AU - Coclite, Anna Maria
AU - Majaron, Boris
AU - Humar, Matjaž
N1 - Funding Information:
This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 851143)(M.H.) and from Slovenian Research Agency (ARRS) (N1-0104 (M.H.), P1-0099 (M.H.) and P1-0192 (B.M.)). We thank Boris Turk, Georgy Mikhaylov, and Petra Matjan Štefin for providing us the biological tissue samples.
Funding Information:
This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 851143)(M.H.) and from Slovenian Research Agency (ARRS) (N1-0104 (M.H.), P1-0099 (M.H.) and P1-0192 (B.M.)). We thank Boris Turk, Georgy Mikhaylov, and Petra Matjan Štefin for providing us the biological tissue samples.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging. Here, we show that optical microcavity probes embedded inside cells enable three-dimensional localization and tracking of individual cells over extended time periods, as well as sensing of their environment, at depths well beyond the light transport length. This is achieved by utilizing unique spectral features of the whispering-gallery modes, which are unaffected by tissue scattering, absorption, and autofluorescence. In addition, microcavities can be functionalized for simultaneous sensing of various parameters, such as temperature or pH value, which extends their versatility beyond the capabilities of standard fluorescent labels.
AB - Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging. Here, we show that optical microcavity probes embedded inside cells enable three-dimensional localization and tracking of individual cells over extended time periods, as well as sensing of their environment, at depths well beyond the light transport length. This is achieved by utilizing unique spectral features of the whispering-gallery modes, which are unaffected by tissue scattering, absorption, and autofluorescence. In addition, microcavities can be functionalized for simultaneous sensing of various parameters, such as temperature or pH value, which extends their versatility beyond the capabilities of standard fluorescent labels.
UR - http://www.scopus.com/inward/record.url?scp=85126246302&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-28904-6
DO - 10.1038/s41467-022-28904-6
M3 - Article
C2 - 35277496
AN - SCOPUS:85126246302
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1269
ER -