TY - JOUR
T1 - Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase
AU - Kracht, Octavia Natascha
AU - Correia Cordeiro, Raquel S.
AU - Håkansson, Maria
AU - Stockmann, Julia
AU - Sander, Dennis
AU - Bandow, Julia
AU - Senges, Christoph H.R.
AU - Logan, Derek T.
AU - Kourist, Robert
PY - 2019/5/20
Y1 - 2019/5/20
N2 - With more than 50,000 members, terpenoids are one of the most important classes of natural products and show an enormous diversity. Due to their unique odors and specific bioactivities they already find wide application in the flavor, fragrance and pharma industries. Since most terpenoids can only be obtained by natural product extraction, the discovery of biosynthetic genes for the generation of terpene diversity becomes increasingly important. This study describes the discovery of three novel sesquiterpene synthases from Streptomyces chartreusis with preference for the formation of germacradiene-11-ol, α-eudesmol and α-amorphene respectively. The α-eudesmol synthase showed formation of 10-epi-δ-eudesmol and elemol as side products. Eudesmol-isomers are known to have repellent activity, which makes this enzyme a potential catalyst for products for the prevention of mosquito-related disease. The determination of the structure of the apo-enzyme of α-eudesmol synthase from S. chartreusis provides the first structural insights into an eudesmol-forming enzyme.
AB - With more than 50,000 members, terpenoids are one of the most important classes of natural products and show an enormous diversity. Due to their unique odors and specific bioactivities they already find wide application in the flavor, fragrance and pharma industries. Since most terpenoids can only be obtained by natural product extraction, the discovery of biosynthetic genes for the generation of terpene diversity becomes increasingly important. This study describes the discovery of three novel sesquiterpene synthases from Streptomyces chartreusis with preference for the formation of germacradiene-11-ol, α-eudesmol and α-amorphene respectively. The α-eudesmol synthase showed formation of 10-epi-δ-eudesmol and elemol as side products. Eudesmol-isomers are known to have repellent activity, which makes this enzyme a potential catalyst for products for the prevention of mosquito-related disease. The determination of the structure of the apo-enzyme of α-eudesmol synthase from S. chartreusis provides the first structural insights into an eudesmol-forming enzyme.
KW - Eudesmol
KW - Insect repellant
KW - Sesquiterpenes
KW - Streptomyces chartreusis
KW - Terpene synthesis
UR - http://www.scopus.com/inward/record.url?scp=85063949753&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2019.03.006
DO - 10.1016/j.jbiotec.2019.03.006
M3 - Article
C2 - 30928538
AN - SCOPUS:85063949753
SN - 0168-1656
VL - 297
SP - 71
EP - 77
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -