TY - JOUR
T1 - Integrating chemical imaging of cationic trace metal solutes and pH into a single hydrogel layer
AU - Hoefer, Christoph
AU - Santner, Jakob
AU - Borisov, Sergey M.
AU - Wenzel, Walter W.
AU - Puschenreiter, Markus
PY - 2017/1/15
Y1 - 2017/1/15
N2 - Gel-based, two-dimensional (2D) chemical imaging techniques are versatile methods for investigating biogeochemically active environments at high spatial resolution (sub-mm). State-of-the-art solute imaging techniques, such as diffusive gradients in thin films (DGT) and planar optodes (PO), employ passive solute sampling or sensing. Combining these methods will provide powerful tools for studying the biogeochemistry of biological niches in soils and sediments. In this study we aimed at developing a combined single-layer gel for direct pH imaging using PO and sampling of anionic and cationic solutes by DGT, with subsequent analysis of the bound solutes by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). We tested three ultra-thin (<100 μm) polyurethane-based gels, incorporating anion and cation binding materials and the fluorescent pH indicator DCIFODA (2′,7′-dichloro-5(6)-N-octadecyl-carboxamidofluorescein). Results showed that PO-based pH sensing using DCIFODA was impossible in the presence of the anion binding materials due to interferences with DCIFODA protonation. One gel, containing only a cation binding material and DCIFODA, was fully characterized and showed similar performance characteristics as comparable DGT-only gels (applicable pH range: pH 5–8, applicable ionic strength range: 1–20 mmol L-1, cation binding capacity ∼24 μg cm−2). The dynamic range for PO-based pH mapping was between pH 5.5 and 7.5 with t90 response time of ∼60 min. In a case study we demonstrated the gel's suitability for multi-analyte solute imaging and mapped pH gradients and concurrent metal solubility patterns in the rhizosphere of Salix smithiana. pH decreases in the rooted soil were co-localized with elevated solute fluxes of Al3+, Co2+, Cu2+, Fe, Mn2+, Ni2+ and Pb2+, indicating pH-induced metal solubilisation.
AB - Gel-based, two-dimensional (2D) chemical imaging techniques are versatile methods for investigating biogeochemically active environments at high spatial resolution (sub-mm). State-of-the-art solute imaging techniques, such as diffusive gradients in thin films (DGT) and planar optodes (PO), employ passive solute sampling or sensing. Combining these methods will provide powerful tools for studying the biogeochemistry of biological niches in soils and sediments. In this study we aimed at developing a combined single-layer gel for direct pH imaging using PO and sampling of anionic and cationic solutes by DGT, with subsequent analysis of the bound solutes by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). We tested three ultra-thin (<100 μm) polyurethane-based gels, incorporating anion and cation binding materials and the fluorescent pH indicator DCIFODA (2′,7′-dichloro-5(6)-N-octadecyl-carboxamidofluorescein). Results showed that PO-based pH sensing using DCIFODA was impossible in the presence of the anion binding materials due to interferences with DCIFODA protonation. One gel, containing only a cation binding material and DCIFODA, was fully characterized and showed similar performance characteristics as comparable DGT-only gels (applicable pH range: pH 5–8, applicable ionic strength range: 1–20 mmol L-1, cation binding capacity ∼24 μg cm−2). The dynamic range for PO-based pH mapping was between pH 5.5 and 7.5 with t90 response time of ∼60 min. In a case study we demonstrated the gel's suitability for multi-analyte solute imaging and mapped pH gradients and concurrent metal solubility patterns in the rhizosphere of Salix smithiana. pH decreases in the rooted soil were co-localized with elevated solute fluxes of Al3+, Co2+, Cu2+, Fe, Mn2+, Ni2+ and Pb2+, indicating pH-induced metal solubilisation.
KW - Chemical imaging
KW - Diffusive gradients in thin films
KW - Fluorescent sensing
KW - Laser ablation inductively coupled plasma mass spectrometry
KW - Planar optode
KW - Rhizosphere
UR - http://www.scopus.com/inward/record.url?scp=84999663648&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2016.11.004
DO - 10.1016/j.aca.2016.11.004
M3 - Article
AN - SCOPUS:84999663648
SN - 0003-2670
VL - 950
SP - 88
EP - 97
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -