Mechanochemical Coupling of Catalysis and Motion in a Cellulose-Degrading Multienzyme Nanomachine

Krisztina Zajki-Zechmeister, Manuel Eibinger, Gaurav Singh Kaira, Bernd Nidetzky*

*Korrespondierende/r Autor/-in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftArtikelBegutachtung

Abstract

The cellulosome is a megadalton-size protein complex that functions as a biological nanomachine of cellulosic fiber degradation. We show that the cellulosome behaves as a Brownian ratchet that rectifies protein motions on the cellulose surface into a propulsion mechanism by coupling to the hydrolysis of cellulose chains. Movement on cellulose fibrils is unidirectional and results from “macromolecular crawl” composed of dynamic switches between elongated and compact spatial arrangements of enzyme subunits. Deletion of the main exocellulase Cel48S eliminates conformational bias for aligning the subunits to the long fibril axis, which we reveal as crucial for optimum coupling between directional movement and substrate degradation. Implications of the cellulosome acting as a mechanochemical motor suggest a distinct mechanism of enzymatic machinery in the deconstruction of cellulose assemblies.

Originalspracheenglisch
Seiten (von - bis)2656-2663
Seitenumfang8
FachzeitschriftACS Catalysis
Jahrgang14
Ausgabenummer4
Frühes Online-Datum6 Feb. 2024
DOIs
PublikationsstatusVeröffentlicht - 16 Feb. 2024

ASJC Scopus subject areas

  • Katalyse
  • Allgemeine Chemie

Fingerprint

Untersuchen Sie die Forschungsthemen von „Mechanochemical Coupling of Catalysis and Motion in a Cellulose-Degrading Multienzyme Nanomachine“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren