TY - JOUR
T1 - Photovoltaic Stimulation Induces Overdrive Suppression in Embryonic Chicken Cardiomyocytes
AU - Polz, Mathias
AU - Ziesel, Daniel
AU - Shrestha, Niroj
AU - Pelzmann, Brigitte
AU - Lang, Petra
AU - Scheruebel, Susanne
AU - Ueçal, Muammer
AU - Kornmueller, Karin
AU - Schröttner, Jörg
AU - Đerek, Vedran
AU - Opančar, Aleksandar
AU - Glowacki, Eric Daniel
AU - Baumgartner, Christian
AU - Rienmüller, Theresa Margarethe
PY - 2023/12/1
Y1 - 2023/12/1
N2 - In this study, we employed calcium imaging to investigate the dynamics of intracellular calcium levels in embryonic chicken cardiomyocytes upon extracellular, optoelectronic stimulation. A photovoltaic layer of donoracceptor pigments on a flexible PET substrate was used as a wireless stimulation electrode. Our findings revealed a distinct change in their spontaneous activity pattern in cardiac cells following asynchronous light stimulation. A short pause in cellular activity, indicative of overdrive suppression, was observed in recordings from several different cells. The pause in activity signifies a transient refractory period induced by stimulation of the photovoltaic device with red light. These findings suggest that photovoltaic electrodes can be used to effectively modulate the electrical activity of cardiac cells in a wireless, non-pharmacological manner. This opens new avenues for non-invasive and precise light-modulated control of cellular electrophysiology as well as potential therapeutic applications for cardiac rhythm disorders.
AB - In this study, we employed calcium imaging to investigate the dynamics of intracellular calcium levels in embryonic chicken cardiomyocytes upon extracellular, optoelectronic stimulation. A photovoltaic layer of donoracceptor pigments on a flexible PET substrate was used as a wireless stimulation electrode. Our findings revealed a distinct change in their spontaneous activity pattern in cardiac cells following asynchronous light stimulation. A short pause in cellular activity, indicative of overdrive suppression, was observed in recordings from several different cells. The pause in activity signifies a transient refractory period induced by stimulation of the photovoltaic device with red light. These findings suggest that photovoltaic electrodes can be used to effectively modulate the electrical activity of cardiac cells in a wireless, non-pharmacological manner. This opens new avenues for non-invasive and precise light-modulated control of cellular electrophysiology as well as potential therapeutic applications for cardiac rhythm disorders.
KW - Arrhythmia
KW - Extracellular Stimulation
KW - OEPC
KW - Optoelectronics
KW - Photocapacitor
UR - http://www.scopus.com/inward/record.url?scp=85180496596&partnerID=8YFLogxK
U2 - 10.1515/cdbme-2023-1204
DO - 10.1515/cdbme-2023-1204
M3 - Article
SN - 2364-5504
VL - 9
SP - 12
EP - 15
JO - Current Directions in Biomedical Engineering
JF - Current Directions in Biomedical Engineering
IS - 2
ER -