siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis

V Grimard; J Massier; Doris Richter; D Schwudke; Y Kalaidzidis; E Fava; Albin Hermetter; C Thiele

doi:10.1194/jlr.M800168-JLR200

siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis

V Grimard, J Massier, Doris Richter, D Schwudke, Y Kalaidzidis, E Fava, Albin Hermetter, C Thiele

Institut für Biochemie (6480)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.

Originalsprache	englisch
Seiten (von - bis)	2427-2440
Fachzeitschrift	Journal of Lipid Research
Jahrgang	49
Ausgabenummer	11
DOIs	https://doi.org/10.1194/jlr.M800168-JLR200
Publikationsstatus	Veröffentlicht - 2008

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10.1194/jlr.M800168-JLR200Lizenz: CC BY-NC-ND 4.0

GEN-AU GOLD II (Genomics of Lipid-associated Disorders): C4 - Functional and differential analysis of lipolytic enzymes by affinity tagging
Schicher, M. (Teilnehmer (Co-Investigator)), Zenzmaier, E. (Teilnehmer (Co-Investigator)), Bobula, T. (Teilnehmer (Co-Investigator)), Lehsl, A. (Teilnehmer (Co-Investigator)), Morak, M. (Teilnehmer (Co-Investigator)), Dunai, Z. (Teilnehmer (Co-Investigator)), Birner-Grünberger, R. (Projektleiter (Principal Investigator)) & Hermetter, A. (Projektleiter (Principal Investigator))
1/12/05 → 30/11/08
Projekt: Forschungsprojekt

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abstract = "Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.",

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AU - Grimard, V

AU - Massier, J

AU - Richter, Doris

AU - Schwudke, D

AU - Kalaidzidis, Y

AU - Fava, E

AU - Hermetter, Albin

AU - Thiele, C

PY - 2008

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AB - Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.

U2 - 10.1194/jlr.M800168-JLR200

DO - 10.1194/jlr.M800168-JLR200

M3 - Article

VL - 49

SP - 2427

EP - 2440

JO - Journal of Lipid Research

JF - Journal of Lipid Research

IS - 11

ER -

siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis

Abstract

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Projekte

GEN-AU GOLD II (Genomics of Lipid-associated Disorders): C4 - Functional and differential analysis of lipolytic enzymes by affinity tagging

Dieses zitieren