Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

Carmen Hermann-Krauss; Martin Koller; Alexander Muhr; Hubert Fasl; Franz Stelzer; Gerhart Braunegg

doi:10.1155/2013/129268

Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

Carmen Hermann-Krauss, Martin Koller^*, Alexander Muhr, Hubert Fasl, Franz Stelzer, Gerhart Braunegg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol.
Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester composition and volumetric productivity,
indicating the feasibility of applying CGP as feedstock. Analysis of molar mass distribution revealed a weight average molar mass𝑀𝑤 of 150 kDa and polydispersity 𝑃𝑖 of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140∘C in both setups. Supplying 𝛾-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-
3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor. The terpolyester displayed reduced melting (melting endotherms at 122 and 137∘C) and glass transition temperature
(2.5∘C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.

Original language	English
Article number	129268
Number of pages	10
Journal	Archaea
Volume	2013
DOIs	https://doi.org/10.1155/2013/129268
Publication status	Published - 2013

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title = "Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products",

abstract = "The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol.Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester composition and volumetric productivity,indicating the feasibility of applying CGP as feedstock. Analysis of molar mass distribution revealed a weight average molar mass𝑀𝑤 of 150 kDa and polydispersity 𝑃𝑖 of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140∘C in both setups. Supplying 𝛾-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor. The terpolyester displayed reduced melting (melting endotherms at 122 and 137∘C) and glass transition temperature(2.5∘C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.",

author = "Carmen Hermann-Krauss and Martin Koller and Alexander Muhr and Hubert Fasl and Franz Stelzer and Gerhart Braunegg",

year = "2013",

doi = "10.1155/2013/129268",

language = "English",

volume = "2013",

journal = "Archaea",

issn = "1472-3654",

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TY - JOUR

T1 - Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

AU - Hermann-Krauss, Carmen

AU - Koller, Martin

AU - Muhr, Alexander

AU - Fasl, Hubert

AU - Stelzer, Franz

AU - Braunegg, Gerhart

PY - 2013

Y1 - 2013

N2 - The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol.Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester composition and volumetric productivity,indicating the feasibility of applying CGP as feedstock. Analysis of molar mass distribution revealed a weight average molar mass𝑀𝑤 of 150 kDa and polydispersity 𝑃𝑖 of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140∘C in both setups. Supplying 𝛾-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor. The terpolyester displayed reduced melting (melting endotherms at 122 and 137∘C) and glass transition temperature(2.5∘C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.

AB - The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol.Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester composition and volumetric productivity,indicating the feasibility of applying CGP as feedstock. Analysis of molar mass distribution revealed a weight average molar mass𝑀𝑤 of 150 kDa and polydispersity 𝑃𝑖 of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140∘C in both setups. Supplying 𝛾-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor. The terpolyester displayed reduced melting (melting endotherms at 122 and 137∘C) and glass transition temperature(2.5∘C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.

U2 - 10.1155/2013/129268

DO - 10.1155/2013/129268

M3 - Article

SN - 1472-3654

VL - 2013

JO - Archaea

JF - Archaea

M1 - 129268

ER -

Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

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