Abstract
OBJECTIVE:
Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-γ (PPARγ) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARγ2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.
DESIGN:
We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h.
MEASUREMENTS:
Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).
RESULTS:
Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.
CONCLUSION:
These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.
Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-γ (PPARγ) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARγ2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.
DESIGN:
We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h.
MEASUREMENTS:
Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).
RESULTS:
Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.
CONCLUSION:
These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.
| Original language | English |
|---|---|
| Pages (from-to) | 934-941 |
| Journal | International Journal of Obesity |
| Volume | 29 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2005 |