Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation

Montserrat Pinent-Armengol, M. Cinta Blade, M. Josepa Salvado, Lluis Arola, Hubert Hackl, John Quackenbush, Zlatko Trajanoski, Anna Ardevol*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review


    Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-γ (PPARγ) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARγ2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.
    We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h.
    Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).
    Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.
    These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.
    Original languageEnglish
    Pages (from-to)934-941
    JournalInternational Journal of Obesity
    Issue number8
    Publication statusPublished - 2005

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