TY - JOUR
T1 - Human leukocyte antigen-G 3’ untranslated region polymorphisms are associated with asthma severity
AU - C. Alves, Cintia
AU - K. P. Arruda, Luisa
AU - R. Oliveira, Fabiola
AU - D. Massaro, Juliana
AU - J. Aquino, Beatriz
AU - Almeida da Paz, Michelle
AU - C. Castelli, Erick
AU - T. Mendes-Junior, Celso
AU - A. Donadi, Eduardo
PY - 2018
Y1 - 2018
N2 - Asthma is a genetically complex chronic inflammatory airway disorder, and according to disease pathogenesis, clinical manifestations may vary according to asthma severity. A gene region close to the human leukocyte antigen-G (HLA-G) gene was identified as an independent susceptibility marker for asthma. Considering that the HLA-G immune checkpoint molecule may modulate inflammation, we evaluated the diversity of the HLA-G 3′ untranslated region (3′UTR) in asthmatic patients stratified according to disease severity. We evaluate the entire HLA-G 3′UTR segment in 115 Brazilian patients stratified into mild (n=29), moderate (n=21) and severe asthmatics (n=65), and in 116 healthy individuals. HLA-G 3′UTR typing was performed using Sanger sequencing. The multiple comparisons among patients stratified according to disease severity revealed several associations; however, after Bonferroni’s correction, the following results remained significant: i) the +3010C and +3142G alleles were overrepresented in mild asthma patients when compared to controls; ii) the +3010G and +3142C alleles were overrepresented in severe asthma patients in comparison to patients with mild asthma. In conclusion, the +3010C/G and +3142C/G HLA-G 3′UTR variation sites were differentially associated according to asthma severity.
AB - Asthma is a genetically complex chronic inflammatory airway disorder, and according to disease pathogenesis, clinical manifestations may vary according to asthma severity. A gene region close to the human leukocyte antigen-G (HLA-G) gene was identified as an independent susceptibility marker for asthma. Considering that the HLA-G immune checkpoint molecule may modulate inflammation, we evaluated the diversity of the HLA-G 3′ untranslated region (3′UTR) in asthmatic patients stratified according to disease severity. We evaluate the entire HLA-G 3′UTR segment in 115 Brazilian patients stratified into mild (n=29), moderate (n=21) and severe asthmatics (n=65), and in 116 healthy individuals. HLA-G 3′UTR typing was performed using Sanger sequencing. The multiple comparisons among patients stratified according to disease severity revealed several associations; however, after Bonferroni’s correction, the following results remained significant: i) the +3010C and +3142G alleles were overrepresented in mild asthma patients when compared to controls; ii) the +3010G and +3142C alleles were overrepresented in severe asthma patients in comparison to patients with mild asthma. In conclusion, the +3010C/G and +3142C/G HLA-G 3′UTR variation sites were differentially associated according to asthma severity.
U2 - 10.1016/j.molimm.2018.08.013
DO - 10.1016/j.molimm.2018.08.013
M3 - Article
SN - 1872-9142
VL - 101
SP - 500
EP - 506
JO - Molecular Immunology
JF - Molecular Immunology
ER -